A study with positron emission tomography has found that healthy subjects that score high on the NEO PI-R neuroticism dimension tend to have high altanserin binding in the frontolimbic region of the brain — an indication that these subjects tend to have more of the 5-HT2A receptor in that location. Another study has found that healthy subjects with a high neuroticism score tend to have higher DASB binding in the thalamus, — with DASB being a ligand that binds to the serotonin transporter protein.
Another neuroimaging study using magnetic resonance imaging to measure brain volume found that the brain volume was negatively correlated to NEO PI-R neuroticism when correcting for possible effects of intracranial volume, sex, and age.
Other studies have associated neuroticism with genetic variations, e.g., with 5-HTTLPR — a polymorphism in the serotonin transporter gene. However, not all studies find such an association. A genome-wide association study (GWA study) has associated single-nucleotide polymorphism in the MDGA2 gene with neuroticism, however the effect sizes were small. Another GWA study gave some evidence that the rs362584 polymorphism in the SNAP25 gene was associated with neuroticism.
Geography - Neuroticism, along with other personality traits, has been mapped across states in the USA. People in eastern states such as New York, New Jersey, West Virginia, and Mississippi tend to score high on neuroticism, whereas people in many western states, such as Utah, Colorado, South Dakota, Oregon, and Arizona score lower on average. People in states that are higher in neuroticism also tend to have higher rates of heart disease and lower life expectancy.